منابع مشابه
Automation of enzyme assays.
The increasing use of enzymes as aids to clinical diagnosis has made it mandatory for clinical biochemists to develop techniques and instrumentation to enable large numbers of specimens to be processed quickly with adequate accuracy. The main objective in enzyme assay is to determine the initial reaction rate, as it is only during this period that there is a linear relationship between the rate...
متن کاملGood preclinical bioanalytical chemistry requires proper sampling from laboratory animals: automation of blood and microdialysis sampling improves the productivity of LC/MSMS.
The preclinical bioanalytical process with animal models begins with sampling biological fluids and tissue. The goal is to understand oral absorption kinetics, distribution, metabolism, excretion, blood brain barrier penetration, drug-drug interactions, and the influences on biomarkers, hematology, electrophysiology, cardiology, blood pressure and behavior. An overview is obtained by periodic b...
متن کاملPharmacokinetic Analysis of the mAb Adalimumab by ELISA and Hybrid LBA/LC/MS: A Comparison Study
The selection of a quantitative protein assay for biologics bioanalysis in a pre-clinical study depends on what platform will provide the right data for the drug under development. Some questions to be considered when choosing an assay platform include, is the total or free drug concentration required? Are there in vivo structural changes that might impact activity? Are these modified proteins ...
متن کاملProgress in automation of LC-MS in laboratory medicine.
BACKGROUND LC-MS/MS is an almost universal technology for the quantification of small molecules in human sample materials. The widespread use of this technology in laboratory medicine is so far limited mainly by the extensive occupation of highly trained personnel which is required for method implementation and application. Furthermore, robustness of function and results is still a critical iss...
متن کاملDevelopment of a method for rapid quantitation of amino acids by liquid chromatography-tandem mass spectrometry (LC-MSMS) in plasma.
A new analytical method has been developed and is proposed for the rapid determination of eighteen common amino acids, including tryptophan, in plasma and dried blood spots, by liquid chromatography coupled with ionspray tandem mass spectrometry. Potentially the method can include other amino acids and can be used for the diagnosis of metabolic disease. The use of the ionspray tandem mass spect...
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ژورنال
عنوان ژورنال: Journal of Applied Bioanalysis
سال: 2017
ISSN: 2405-710X
DOI: 10.17145/jab.17.005